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BD Stemflow 人 MSC 分析試劑盒 562245

人多能間充質(zhì)基質(zhì)細(xì)胞分析試劑盒,BD Stemflow™ Human MSC Analysis Kit

描述：

細(xì)胞特性與鑒定標(biāo)準(zhǔn)

人源多能間充質(zhì)基質(zhì)細(xì)胞(Mesenchymal Stromal Cells, MSCs)，亦稱間充質(zhì)干細(xì)胞，是一類具有以下特征的成體干細(xì)胞：

• 來源多樣性：可從骨髓、脂肪等多種組織中分離獲得

• 多向分化潛能：經(jīng)體外培養(yǎng)可分化為脂肪細(xì)胞(adipocytes)、骨細(xì)胞(osteocytes)和軟骨細(xì)胞(chondrocytes)

• 免疫調(diào)節(jié)功能：在體內(nèi)外均表現(xiàn)出顯著的免疫調(diào)控活性

根據(jù)國(guó)際細(xì)胞治療協(xié)會(huì)(ISCT)2006年制定的標(biāo)準(zhǔn)，骨髓來源MSCs應(yīng)滿足：

陽(yáng)性標(biāo)志物：CD73(+) | CD90(+) | CD105(+)
陰性標(biāo)志物：CD34(-) | CD45(-) | CD11b/CD14(-) | CD19/CD79α(-) | HLA-DR(-)
其他常見標(biāo)志物：CD44/CD29/CD200/CD166/CD146/CD271等

試劑盒技術(shù)創(chuàng)新

1. 標(biāo)準(zhǔn)化檢測(cè)方案

• 完整包含ISCT推薦的核心標(biāo)志物組合

• 陽(yáng)性混合液(FITC CD90 + PerCP-Cy™5.5 CD105 + APC CD73)

• 陰性混合液(PE CD45/CD34/CD11b/CD19/HLA-DR)

2. 模塊化設(shè)計(jì)優(yōu)勢(shì)

• 試劑盒內(nèi)附PE-CD44抗體

• 其他市售PE標(biāo)記抗體

• 保留PE通道靈活性，可兼容：

• 支持用戶自定義擴(kuò)展檢測(cè)項(xiàng)目

3. 實(shí)驗(yàn)效率優(yōu)化

• 多色流式技術(shù)降低樣本需求量

• 預(yù)混抗體組合簡(jiǎn)化操作流程

• 單獨(dú)陽(yáng)性對(duì)照抗體便于補(bǔ)償調(diào)節(jié)

注：本方案適用于流式細(xì)胞術(shù)分析，建議配合同型對(duì)照設(shè)置和熒光補(bǔ)償對(duì)照使用，以獲得優(yōu)良檢測(cè)結(jié)果。

BD Stemflow 人 MSC 分析試劑盒 562245

Human multipotent mesenchymal stromal cells (MSCs), also referred to as mesenchymal stem cells, are a rare population of adult stem cells that can be isolated from a variety of tissues.  MSCs that have been isolated from bone marrow and subsequently cultured can differentiate to a variety of cell types, most notably adipocytes, osteocytes and chondrocytes.  MSCs also have immunomodulatory effects in vivo and in vitro.  In 2006, the International Society for Cellular Therapy (ISCT) proposed a cell surface marker panel for the minimal identification of human MSCs derived from bone marrow.  Under this recommendation MSCs should be positive for CD73, CD90, and CD105, but be negative for CD34, CD45, CD11b or CD14, CD19 or CD79α, and HLA-DR.  MSCs are also known to express numerous cell surface markers such as CD44, CD29, CD200, CD166, CD146 and CD271.  In this kit we include the panel that was proposed by the ISCT.  In addition, we designed this kit to be modular so that additional markers could be "dropped-in".  Specifically, the MSC positive cocktail (FITC CD90, PerCP-Cy™5.5 CD105 and APC CD73) leaves the PE channel open to use in combination with the supplied negative MSC cocktail (PE CD45, PE CD34, PE CD11b, PE CD19 and PE HLA-DR), the included PE CD44 antibody conjugate, or a variety of other commercially available PE antibody conjugates.  Multi color analysis minimizes cell numbers required for an assay and antibody cocktails facilitate a streamlined staining protocol to analyze multiple samples.  Individual positive markers are included for compensation set-up.

準(zhǔn)備和存儲(chǔ)

在 4°C 下原液保存，避免長(zhǎng)時(shí)間光照。不要凍結(jié)。